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Eiji Kano Onsen Trip Download HD - · 2014 · 3dgarden: Eiji in Onsen Trip – Just too cute. kanoeiji-ia reblogged this from xxxeinsamxengelxxx .
Eiji in Onsen Trip (Yuuki Onsen + Eiji Kano + T-san) –
Eiji in Onsen Trip (Okada Yuuki + T-san) – facets of miR-138 and miR-146a-mediated protective outcomes in sepsis.
Sepsis continues to be a major cause of morbidity and mortality in the ICU. The role of microRNAs (miRNAs) in sepsis is currently under investigation. A conserved miRNA, miR-138, has recently been shown to be a critical regulator of proinflammatory cytokine production in response to LPS and lipoteichoic acid in macrophages. The goal of this study was to further elucidate the protective effects of miR-138 in sepsis and identify its role in a clinically relevant model of endotoxemia. miR-138 was transfected into RAW264.7 cells prior to LPS stimulation, and cytokines secreted from the cells were measured by enzyme-linked immunosorbent assay (ELISA). miR-138 knockdown was used to investigate the effect of miR-138 overexpression in sepsis. Effects of miR-138 on the TLR4 signaling pathway and phagocytic function of macrophages were measured using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), Western blotting, flow cytometry, and NBT and Cys-X-Cy7 labeling. In addition, knockdown and overexpression of miR-138 in the peritoneal macrophages of septic mice were used to investigate the role of miR-138 in sepsis. Our results demonstrated that miR-138 transfection reduced LPS-induced proinflammatory cytokine production by macrophages, whereas miR-138 knockdown enhanced the production of these cytokines. mi
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